Photosynthesis in Higher Plants MCQ – Best 100 MCQs. These 100 Multiple Choice Questions with Answer and Explanation will help you score full marks in your class 11 Biology exam.
Photosynthesis in Higher Plants MCQ – Best 100 MCQs
What Do We Know about Photosynthesis in Higher Plants?
Question 1: Which of the following is NOT a basic requirement for photosynthesis?
A. Chlorophyll
B. Light
C. Oxygen
D. Carbon dioxide
Question 2: The presence of starch in a leaf indicates that:
A. The leaf has been exposed to light.
B. The leaf contains chlorophyll.
C. Photosynthesis has occurred.
D. All of the above.
Question 3: In a variegated leaf experiment, which part of the leaf would test positive for starch after exposure to light?
A. The green parts
B. The non-green parts
C. Both green and non-green parts
D. Neither green nor non-green parts
Early Experiments – Photosynthesis in Higher Plants MCQs
Question 4: Which scientist is credited with discovering oxygen in 1774?
A. Joseph Priestley
B. Jan Ingenhousz
C. Julius von Sachs
D. Cornelius van Niel
Question 5: Ingenhousz’s experiments demonstrated that:
A. Plants restore to the air whatever breathing animals and burning candles remove.
B. Sunlight is essential for plants to purify the air.
C. The green parts of plants release oxygen in the presence of sunlight.
D. Glucose is produced and stored as starch during plant growth.
Question 6: Julius von Sachs’s studies revealed that:
A. Photosynthesis is a light-dependent reaction.
B. The oxygen evolved during photosynthesis comes from water.
C. Glucose is produced during photosynthesis and stored as starch.
D. The first CO2 fixation product is a 3-carbon organic acid.
Question 7: Cornelius van Niel’s research on purple and green bacteria led to the understanding that:
A. Photosynthesis primarily occurs in the blue and red regions of the light spectrum.
B. Photosynthesis is a process where hydrogen from a suitable compound reduces carbon dioxide to carbohydrates.
C. The oxygen released during photosynthesis comes from carbon dioxide.
D. The first stable product of CO2 fixation in some plants is a 4-carbon organic acid.
Where Does Photosynthesis Take Place? – Photosynthesis in Higher Plants MCQ
Question 8: The primary sites of photosynthesis in plants are:
A. Green leaves only
B. Roots and stems
C. Green leaves and other green parts of the plant
D. Flowers and fruits
Question 9: Which cells in leaves are specialized for photosynthesis?
A. Epidermal cells
B. Guard cells
C. Mesophyll cells
D. Xylem cells
Question 10: The internal membrane system within chloroplasts is organized into:
A. Grana, stroma lamellae, and stroma
B. Thylakoids, cristae, and matrix
C. Vacuoles, cytoplasm, and nucleus
D. Cell wall, plasma membrane, and endoplasmic reticulum
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Question 11: Light reactions of photosynthesis involve:
A. The synthesis of sugars
B. The fixation of carbon dioxide
C. Light absorption, water splitting, and ATP and NADPH formation
D. The release of carbon dioxide
Question 12: Dark reactions (carbon reactions) of photosynthesis:
A. Occur only in the absence of light
B. Are independent of the products of light reactions
C. Utilize ATP and NADPH to synthesize sugars
D. Release oxygen
How Many Pigments are Involved in Photosynthesis? – Photosynthesis in Higher Plants MCQ
Question 13: The color of leaves is due to:
A. A single pigment called chlorophyll
B. A combination of four pigments: chlorophyll a, chlorophyll b, xanthophylls, and carotenoids
C. The presence of anthocyanins
D. The reflection of all wavelengths of light
Question 14: Paper chromatography is used to:
A. Measure the rate of photosynthesis
B. Separate and identify the different pigments present in leaves
C. Determine the absorption spectrum of chlorophyll a
D. Study the structure of chloroplasts
Question 15: The most abundant plant pigment in the world is:
A. Chlorophyll a
B. Chlorophyll b
C. Xanthophylls
D. Carotenoids
Question 16: The absorption spectrum of chlorophyll a shows maximum absorption in the:
A. Green and yellow regions
B. Blue and red regions
C. Violet and orange regions
D. Infrared region
Question 17: The action spectrum of photosynthesis:
A. Shows the wavelengths of light at which maximum photosynthesis occurs
B. Is identical to the absorption spectrum of chlorophyll a
C. Indicates that only chlorophyll a is involved in photosynthesis
D. Demonstrates that plants absorb all wavelengths of light equally
Question 18: Accessory pigments like chlorophyll b, xanthophylls, and carotenoids:
A. Play no role in photosynthesis
B. Absorb light and transfer the energy to chlorophyll a
C. Protect chlorophyll a from photo-oxidation
D. Both B and C
What is Light Reaction? – Photosynthesis in Higher Plants MCQ
Question 19: The light reaction of photosynthesis involves:
A. The synthesis of sugars from carbon dioxide and water
B. The fixation of carbon dioxide into organic molecules
C. Light absorption, water splitting, oxygen release, and the formation of ATP and NADPH
D. The breakdown of glucose to release energy
Question 20: Photosystems I and II are:
A. Protein complexes involved in the electron transport chain
B. Light-harvesting complexes containing pigments
C. Reaction centers where light energy is converted into chemical energy
D. All of the above
Question 21: The reaction center chlorophyll a in photosystem I (PS I) is called P700 because:
A. It absorbs light with a wavelength of 700 nm
B. It is the 700th chlorophyll a molecule in the photosystem
C. It has a molecular weight of 700
D. It was discovered in the year
Question 22: The Z scheme in photosynthesis represents:
A. The absorption spectra of different pigments
B. The flow of electrons during the light reactions
C. The Calvin cycle
D. The structure of a chloroplast
Question 23: The primary electron donor in photosystem II (PS II) is:
A. Water
B. Carbon dioxide
C. Chlorophyll a
D. NADPH
Question 24: The final electron acceptor in the non-cyclic electron transport chain is:
A. Oxygen
B. NADP+
C. ATP
D. Carbon dioxide
Question 25: The movement of electrons through the electron transport chain is driven by:
A. Differences in redox potential
B. ATP hydrolysis
C. Light energy
D. Concentration gradients
Question 26: The energy released during electron transport is used to:
A. Split water molecules
B. Generate a proton gradient
C. Reduce NADP+ to NADPH
D. All of the above
Question 27: In photosystem I (PS I), the reaction center chlorophyll a:
A. Absorbs light at 680 nm
B. Is called P680
C. Absorbs light at 700 nm
D. Is the primary electron donor
Question 28: During non-cyclic photophosphorylation:
A. Only ATP is synthesized
B. Only NADPH is synthesized
C. Both ATP and NADPH are synthesized
D. Neither ATP nor NADPH is synthesized
Question 29: Cyclic photophosphorylation:
A. Involves both PS I and PS II
B. Occurs in the grana lamellae
C. Generates only ATP
D. Produces NADPH
Question 30: The conditions that favor cyclic photophosphorylation include:
A. Low light intensity
B. High light intensity
C. Presence of only wavelengths beyond 680 nm
D. Both A and C
Question 31: The primary function of the electron transport chain in photosynthesis is to:
A. Generate oxygen
B. Fix carbon dioxide
C. Transfer energy from light to ATP and NADPH
D. Synthesize glucose
Question 32: The Z scheme gets its name from:
A. The shape of the electron transport chain when plotted on a redox potential scale
B. The zigzag movement of electrons between photosystems
C. The involvement of zinc ions in the process
D. The discovery of the pathway by a scientist named Z
Splitting of Water – Photosynthesis in Higher Plants MCQ
Question 33: Water splitting is associated with:
A. Photosystem I
B. Photosystem II
C. Both photosystems I and II
D. Neither photosystem
Question 34: The products of water splitting are:
A. Protons, electrons, and carbon dioxide
B. Protons, electrons, and oxygen
C. Oxygen, carbon dioxide, and ATP
D. ATP, NADPH, and oxygen
Question 35: The electrons released during water splitting:
A. Are used to reduce NADP+ to NADPH
B. Replace the electrons lost from the reaction center chlorophyll in PS II
C. Are transferred to PS I
D. Combine with carbon dioxide to form glucose
Question 36: The oxygen released during photosynthesis originates from:
A. Carbon dioxide
B. Water
C. Glucose
D. Chlorophyll
Cyclic and Non-cyclic Photo-phosphorylation – Photosynthesis in Higher Plants MCQ
Question 37: The process of synthesizing ATP from ADP and inorganic phosphate in the presence of light is called:
A. Oxidative phosphorylation
B. Substrate-level phosphorylation
C. Photophosphorylation
D. Chemiosmosis
Question 38: The key difference between cyclic and non-cyclic photophosphorylation lies in:
A. The involvement of photosystems
B. The production of ATP
C. The production of NADPH
D. All of the above
Question 39: Which photosystem is involved in both cyclic and non-cyclic photophosphorylation?
A. Photosystem I
B. Photosystem II
C. Both photosystems I and II
D. Neither photosystem
Question 40: The primary location for cyclic photophosphorylation is:
A. Grana lamellae
B. Stroma lamellae
C. Stroma
D. Thylakoid lumen
Question 41: Which of the following conditions favors cyclic photophosphorylation?
A. High light intensity
B. Abundance of NADPH
C. Wavelengths of light beyond 680 nm
D. Low ATP levels
Question 42: The main advantage of cyclic photophosphorylation is:
A. Production of both ATP and NADPH
B. Increased efficiency of light energy capture
C. Additional ATP synthesis to meet the demands of the Calvin cycle
D. Oxygen evolution
Chemiosmotic Hypothesis – Photosynthesis in Higher Plants MCQ
Question 43: The chemiosmotic hypothesis explains the mechanism of:
A. ATP synthesis in chloroplasts
B. Water splitting
C. Electron transport
D. Carbon fixation
Question 44: The proton gradient across the thylakoid membrane is created by:
A. Water splitting on the inner side of the membrane
B. Proton pumping during electron transport
C. Removal of protons from the stroma during NADP+ reduction
D. All of the above
Question 45: The breakdown of the proton gradient leads to:
A. ATP synthesis
B. Water splitting
C. NADPH formation
D. Carbon fixation
Question 46: ATP synthase consists of two parts:
A. CF0 and CF1
B. PS I and PS II
C. LHC I and LHC II
D. RuBisCO and PEPcase
Question 47: The energy for ATP synthesis is provided by:
A. The breakdown of the proton gradient
B. Light energy directly
C. The oxidation of glucose
D. The reduction of NADP+
Question 48: Chemiosmosis in chloroplasts differs from that in mitochondria in terms of:
A. The direction of proton pumping
B. The involvement of ATP synthase
C. The source of energy for proton pumping
D. All of the above
Where are the ATP and NADPH Used? – Photosynthesis in Higher Plants MCQ
Question 49: The biosynthetic phase of photosynthesis, also known as the dark reaction, primarily involves:
A. The absorption of light energy
B. The splitting of water molecules
C. The synthesis of sugars using ATP and NADPH
D. The release of oxygen
Question 50: Melvin Calvin’s work with radioactive 14C led to the discovery that the first stable product of CO2 fixation in photosynthesis is:
A. Glucose
B. RuBP
C. PGA (3-phosphoglyceric acid)
D. OAA (oxaloacetic acid)
Question 51: The distinction between C3 and C4 plants is based on:
A. The presence or absence of chlorophyll
B. The type of primary CO2 acceptor
C. The first stable product of CO2 fixation
D. The ability to perform photosynthesis
The Primary Acceptor of CO2 – Photosynthesis in Higher Plants MCQ
Question 52: The primary acceptor of CO2 in the Calvin cycle is:
A. RuBP (ribulose bisphosphate)
B. PEP (phosphoenolpyruvate)
C. PGA (3-phosphoglyceric acid)
D. OAA (oxaloacetic acid)
Question 53: The identification of RuBP as the primary CO2 acceptor was:
A. A straightforward process
B. Initially assumed to be a 2-carbon compound
C. A result of Melvin Calvin’s experiments
D. Both B and C
Question 54: The enzyme responsible for catalyzing the reaction between CO2 and RuBP is:
A. RuBisCO
B. PEP carboxylase
C. ATP synthase
D. NADP reductase
The Calvin Cycle – Photosynthesis in Higher Plants MCQ
Question 55: The Calvin cycle can be divided into three stages:
A. Carboxylation, reduction, and regeneration
B. Light absorption, electron transport, and ATP synthesis
C. Glycolysis, Krebs cycle, and oxidative phosphorylation
D. Carbon fixation, sugar synthesis, and oxygen release
Question 56: During the carboxylation stage of the Calvin cycle:
A. CO2 is fixed to RuBP, forming two molecules of PGA
B. ATP and NADPH are used to reduce PGA to G3P
C. RuBP is regenerated from G3P
D. Glucose is synthesized
Question 57: The reduction stage of the Calvin cycle involves:
A. The fixation of CO2 to RuBP
B. The conversion of PGA to G3P using ATP and NADPH
C. The regeneration of RuBP
D. The release of oxygen
Question 58: The regeneration stage of the Calvin cycle is essential for:
A. The continuation of the cycle
B. The synthesis of glucose
C. The fixation of carbon dioxide
D. The release of oxygen
Question 59: How many turns of the Calvin cycle are required to produce one molecule of glucose?
A. 1
B. 3
C. 6
D. 12
Question 60: The enzyme RuBisCO is unique because:
A. It is the most abundant enzyme on Earth
B. It can bind to both CO2 and O2
C. It catalyzes the first step of the Calvin cycle
D. All of the above
Question 61: The overall input of the Calvin cycle includes:
A. Six CO2, 18 ATP, and 12 NADPH
B. One glucose, 18 ADP, and 12 NADP+
C. Six CO2, 12 ATP, and 18 NADPH
D. One glucose, 12 ADP, and 18 NADP+
Question 62: The overall output of the Calvin cycle includes:
A. Six CO2, 18 ATP, and 12 NADPH
B. One glucose, 18 ADP, and 12 NADP+
C. Six CO2, 12 ATP, and 18 NADPH
D. One glucose, 12 ADP, and 18 NADP+
Question 63: For each CO2 molecule entering the Calvin cycle, how many ATP and NADPH molecules are required?
A. 1 ATP and 1 NADPH
B. 2 ATP and 2 NADPH
C. 3 ATP and 2 NADPH
D. 2 ATP and 3 NADPH
Question 64: The Calvin cycle occurs in:
A. All photosynthetic plants
B. Only C3 plants
C. Only C4 plants
D. Neither C3 nor C4 plants
The C4 Pathway – Photosynthesis in Higher Plants MCQ
Question 65: C4 plants are adapted to:
A. Cold, high-altitude environments
B. Dry, tropical regions
C. Temperate forests
D. Aquatic habitats
Question 66: Which of the following is NOT a characteristic of C4 plants?
A. Special leaf anatomy (Kranz anatomy)
B. Tolerance to high temperatures
C. High rates of photorespiration
D. Greater productivity of biomass
Question 67: The primary CO2 acceptor in C4 plants is:
A. RuBP
B. PEP
C. PGA
D. OAA
Question 68: The enzyme responsible for fixing CO2 to PEP in C4 plants is:
A. RuBisCO
B. PEP carboxylase
C. ATP synthase
D. NADP reductase
Question 69: The 4-carbon compound formed in mesophyll cells of C4 plants is transported to bundle sheath cells in the form of:
A. Malic acid or aspartic acid
B. Pyruvate
C. Glucose
D. RuBP
Question 70: In C4 plants, the Calvin cycle takes place in:
A. Mesophyll cells
B. Bundle sheath cells
C. Both mesophyll and bundle sheath cells
D. Neither mesophyll nor bundle sheath cells
Question 71: Bundle sheath cells in C4 plants are characterized by:
A. A large number of chloroplasts
B. Thick walls impervious to gaseous exchange
C. No intercellular spaces
D. All of the above
Question 72: The 3-carbon molecule released after the breakdown of C4 acids in bundle sheath cells is:
A. PEP
B. Pyruvate
C. Glucose
D. RuBP
Question 73: Kranz anatomy refers to:
A. The arrangement of mesophyll cells around the vascular bundle sheath in C4 plants
B. The structure of chloroplasts in C3 plants
C. The presence of large vacuoles in plant cells
D. The arrangement of stomata on leaves
Question 74: The main advantage of the C4 pathway over the C3 pathway is:
A. Increased water loss
B. Reduced light absorption
C. Minimized photorespiration and enhanced CO2 fixation under high temperatures
D. Lower biomass productivity
Photorespiration – Photosynthesis in Higher Plants MCQ
Question 75: Photorespiration is a process where:
A. RuBisCO binds to CO2 and initiates the Calvin cycle
B. RuBisCO binds to O2, leading to the breakdown of RuBP and release of CO2
C. ATP and NADPH are synthesized
D. Glucose is produced
Question 76: In C3 plants, photorespiration is:
A. Absent
B. More likely to occur at low light intensities
C. More likely to occur at high CO2 concentrations
D. More likely to occur at high temperatures and low CO2 concentrations
Question 77: C4 plants avoid photorespiration by:
A. Increasing the concentration of CO2 at the RuBisCO enzyme site
B. Decreasing the concentration of O2 in bundle sheath cells
C. Using PEP carboxylase instead of RuBisCO for initial CO2 fixation
D. All of the above
Question 78: The biological function of photorespiration is:
A. Well understood
B. To generate additional ATP
C. To release excess oxygen
D. Not yet fully known
Question 79: Compared to C3 plants, C4 plants:
A. Have higher rates of photorespiration
B. Are less productive
C. Are more tolerant to high temperatures
D. Are better adapted to cold climates
Question 80: The oxygenase activity of RuBisCO:
A. Is beneficial for photosynthesis
B. Increases CO2 fixation
C. Leads to the formation of PGA and phosphoglycolate
D. Occurs only in C4 plants
Factors Affecting Photosynthesis – Photosynthesis in Higher Plants MCQ
Question 81: The rate of photosynthesis is influenced by:
A. Internal factors (plant-related)
B. External factors (environmental)
C. Both internal and external factors
D. Neither internal nor external factors
Question 82: Which of the following is an internal factor affecting photosynthesis?
A. The amount of chlorophyll
B. Temperature
C. Carbon dioxide concentration
D. Sunlight availability
Question 83: Blackman’s Law of Limiting Factors states that:
A. The rate of a process is determined by the factor present in the highest amount
B. The rate of a process is determined by the factor nearest to its minimal value
C. All factors affect a process equally
D. External factors are more important than internal factors
Light – Photosynthesis in Higher Plants MCQ
Question 84: The intensity of light at which the rate of photosynthesis no longer increases is called the:
A. Compensation point
B. Saturation point
C. Light optimum
D. Light maximum
Question 85: Light saturation in plants typically occurs at:
A. 1% of full sunlight
B. 10% of full sunlight
C. 50% of full sunlight
D. 100% of full sunlight
Question 86: Excessive light can lead to:
A. Increased photosynthesis
B. Breakdown of chlorophyll
C. Enhanced CO2 fixation
D. Increased plant growth
Carbon dioxide Concentration – Photosynthesis in Higher Plants MCQ
Question 87: The current concentration of CO2 in the atmosphere is:
A. Limiting for C3 plants
B. Limiting for C4 plants
C. Optimal for both C3 and C4 plants
D. Damaging to plants
Question 88: C4 plants show saturation of CO2 fixation at a _____ concentration compared to C3 plants.
A. Lower
B. Higher
C. Same
D. Variable
Question 89: CO2 enrichment is sometimes used in greenhouses to:
A. Increase crop yields
B. Reduce photorespiration
C. Decrease water loss
D. Control pests
Temperature – Photosynthesis in Higher Plants MCQ
Question 90: The dark reactions of photosynthesis are:
A. Temperature-independent
B. Temperature-controlled due to their enzymatic nature
C. More sensitive to temperature than light reactions
D. Less sensitive to temperature than light reactions
Question 91: C4 plants have a _____ temperature optimum for photosynthesis compared to C3 plants.
A. Lower
B. Higher
C. Same
D. Variable
Water – Photosynthesis in Higher Plants MCQ
Question 92: Water stress affects photosynthesis primarily by:
A. Directly inhibiting the light reactions
B. Causing stomatal closure and reducing CO2 availability
C. Increasing the rate of photorespiration
D. Damaging chloroplasts
Question 93: Besides affecting CO2 availability, water stress also:
A. Increases leaf surface area
B. Enhances metabolic activity
C. Causes leaves to wilt, reducing their surface area and metabolic activity
D. Promotes chlorophyll synthesis
Question 94: The effect of water as a factor on photosynthesis is mainly:
A. Direct, by influencing the light reactions
B. Indirect, through its impact on stomatal opening and leaf physiology
C. Negligible, as water is abundant in most environments
D. Primarily related to its role as a reactant in the dark reactions
Question 95: The optimal temperature for photosynthesis:
A. Is the same for all plants
B. Varies depending on the plant’s habitat and adaptations
C. Is always higher for C4 plants than C3 plants
D. Is not affected by the type of photosynthesis (C3 or C4)
Question 96: Which of the following statements about the effect of light on photosynthesis is FALSE?
A. At low light intensities, there is a linear relationship between light intensity and CO2 fixation rate.
B. Light saturation occurs at 100% of full sunlight.
C. Beyond the saturation point, further increases in light intensity do not increase the rate of photosynthesis.
D. Excessive light can damage chlorophyll and decrease photosynthesis.
Question 97: The main limiting factor for photosynthesis in natural environments is usually:
A. Light
B. Carbon dioxide concentration
C. Temperature
D. Water
Question 98: Which of the following is NOT an external factor affecting photosynthesis?
A. Temperature
B. Carbon dioxide concentration
C. The number of chloroplasts
D. Water availability
Question 99: When a plant experiences water stress:
A. The stomata open wider to allow more CO2 uptake
B. The rate of photosynthesis increases
C. The leaves wilt, reducing their surface area and metabolic activity
D. The plant switches from C3 to C4 photosynthesis
Question 100: The primary way water influences photosynthesis is by:
A. Acting as a reactant in the light reactions
B. Affecting the plant’s ability to regulate CO2 uptake and maintain leaf function
C. Directly influencing the activity of RuBisCO
D. Providing a medium for nutrient transport
Can you tell if a plant is C3 or C4 just by looking at it?
No, not really. They can look pretty similar on the outside. Sometimes C4 plants have thicker leaves because they live in hot, dry places, but that’s not a sure thing. You need to peek inside the leaf to be sure.
What’s the inside scoop on telling C3 and C4 plants apart?
It’s all about how the cells are arranged near the leaf veins. C4 plants have a special pattern called Kranz anatomy, with big bundle sheath cells hugging the veins. C3 plants don’t have this.
C4 plants are super productive, but only a few of their cells do the Calvin cycle. How does that work?
C4 plants are masters of teamwork! Their mesophyll cells are like CO2 collectors, grabbing it and sending it to the bundle sheath cells. This is where the Calvin cycle happens, making sugars without the energy-wasting photorespiration that slows down C3 plants.
RuBisCO has two jobs: fixing CO2 (good) and using O2 (not so good). Why is it better at its good job in C4 plants?
C4 plants pump up the CO2 levels around RuBisCO. With so much CO2 around, RuBisCO is more likely to do its main job of fixing carbon instead of messing with oxygen.
Could a plant with lots of chlorophyll b but no chlorophyll a do photosynthesis? Why have chlorophyll b at all then?
No way! Chlorophyll a is the star of the show, turning light into energy. Chlorophyll b and other pigments are like backup singers, helping capture more light and passing the energy to chlorophyll a.
Why do leaves in the dark turn yellow or pale green?
Chlorophyll, the green pigment, needs light to stay healthy. In the dark, it breaks down, and you see the other pigments that were always there, like yellow carotenoids.
Which leaves are darker green: those in the shade or those in the sun?
Sunny leaves are darker green. They make more chlorophyll to soak up as much light as possible since there’s less of it in the shade.
C3 Vs C4 pathways
C3 plants make a 3-carbon molecule first when fixing CO2, while C4 plants make a 4-carbon one. C4 plants are better in hot, dry places because they avoid photorespiration.
Cyclic Vs non-cyclic photophosphorylation
Cyclic photophosphorylation only makes ATP, using just Photosystem I. Non-cyclic uses both photosystems and makes ATP and NADPH.
Leaf anatomy in C3 Vs C4 plants
C3 leaves have mesophyll cells doing all the work. C4 leaves have special bundle sheath cells around the veins where the Calvin cycle happens, keeping it away from the CO2-collecting mesophyll cells